Bøger i Methods in Enzymology serien

Major topics covered in this volume cover new reagents and approaches to caged molecules, as well as applications in cell physiology and structure-function relationships.

This volume covers neoglycoconjugates. They are powerful reagents in many cell biology studies and excellent tools for the isolation and characterization of animal and plant lectins, separation of cells, as well as for the targeting of drugs, artificial vaccines and diagnostic reagents.

A history of Israel's Defence Force describing the conditions that forced it to develop skilled fighters and analyzing the long conflict with the Arabs which in 1967 resulted in the Six-Day War.

A history of Israel's Defence Force describing the conditions that forced it to develop skilled fighters and analyzing the long conflict with the Arabs which in 1967 resulted in the Six-Day War.

Neoglycoconjugates are not only useful for the basic understanding of protein-carbohydrate interactions, but they have many practical applications as well. They are powerful reagents in many cell biology studies and excellent tools for the isolation of plant and animal lectins.

This volume provides a methodology for the purification and characterization of the enzymes involved in phospholipid biosynthesis and for the generation of phospholipid biosynthetic mutants, and information on the mechanisms utilized by the cells for the transfer of phospholipids.

Providing up-to-date, state-of-the-art methods for determining the metal content of metalloenzymes, metalloproteins, and other metallobiochemicals, this text emphasizes the need for control of contamination, as well as techniques for handling and dealing with contamination-sensitive molecules.

This volume on phospholipases presents techniques for the assay, purification, and characterization of these enzymes, which are not only degradative enzymes of lipid metabolism but also key enzymes in the signal transduction pathway.

Includes molecular, biological and biochemical methods for the study of cell surface heterotrimeric G proteins. Methods unique to signal transducing G proteins and general techniques applied to the study of G protein systems are covered. The companion issue, volume 238, covers G protein effectors.

This first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and assays for secreted ribonucleases; viral ribonucleases, artificial and engineered ribonucleases, and