Bøger i Neuromethods serien

There are detailed methods on leading techniques for stem cell genetic engineering and intra-organ transplantation for research and therapeutic purposes, in vivo imaging of donor stem cells, and essential requirements for setting up a new stem cell laboratory.

This volume explores current viewpoints and knowledge gaps in the field of traumatic brain injury (TBI). The chapters in this book cover topics ranging from development of in vitro and animal TBI models, to diagnostic imaging and disease monitoring in patients. Designing pre-clinical and clinical trials is also discussed. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Practical and thorough, Pre-Clinical and Clinical Methods in Brain Trauma Research is a valuable resource for both scientists and clinical researchers interested in learning about important techniques and their applications in the field of TBI.

This volume provides properties, biological function, methods,and approaches for manipulating nAChRs in different organisms. NicotinicAcetylcholine Receptor Technologies guides readers through moleculartechniques and behavioral tests used to investigate nicotinic drugs,chronoamperometry, emerging technologies and methods for the analysis ofnAChRs, single nucleotide polymorphism (SNP), fluorescence techniques, spectralconfocal, allosteric modulators of a7-nAChRs, and a comprehensive evolutionalrelation for most nAChR subunits, in both vertebrate and invertebrate species.Written for the popular Neuromethods series, chapters include thekind of detail and key implementation advice that ensures successful results inthe laboratory. Authoritative and practical, Nicotinic AcetylcholineReceptor Technologies aims to ensure successful results in thefurther study of this vital field.

This volume describes a broad spectrum of experimental approaches for investigating structure, function, and transport of neuronal mitochondria in health and disease. Most of these approaches were only recently developed and range from electron tomography-based 3D reconstruction of mitochondrial cristae to patch clamp recording from mitochondria in intact neurons. The chapters in this book cover topics such as mitochondrial proteomics, fluorescence lifetime imaging, respirometry and mitophagy, as well as optical approaches based on the use of genetically engineered fluorescent sensors for monitoring synaptic ATP and axonal ROS generation, mitochondrial Ca2+ cycling and pH changes, and mitochondrial dynamics and axonal trafficking in live neurons. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Techniques to Investigate Mitochondrial Function in Neurons is a valuable and useful resource for a broad range of investigators interested in the function of neuronal mitochondria in health and disease states.

This volume focuses on protein analysis covering a wide spectrum of the utility of mass spectrometry within neurobiological disciplines. The chapters in this book discuss label (iTRAQ, TMT, protein arrays) and label-free workflows (SWATH, MALDI Imaging, and label-free quantitation). Other chapters look at experimental strategies targeted to the identification and quantitation of specific lipids and post-translational modifications, as well as proteomic workflows that focus on characterization of subcellular proteomes. The last few chapters in the book describe various bioinformatics pipelines used to analyze the molecular data derived from high-throughput transcriptomic and proteomic experiments on brain tissue. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.Thorough and comprehensive, Current Proteomic Approaches Applied to Brain Function is a valuable resource for graduate students and postdoctoral fellows interested in neuroproteomics, as well as researchers looking for further insight into the growing field of mass spectrometry in neuroscience.

This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience.

This volume explores both simple and sophisticated techniques used in the study of different types of lateralization of brain and behavior. Part II covers neurobiological methods used to reveal lateralization, such as lesion studies, electrophysiology and pharmacology, early gene expression, and new optogenetic methods.

This volume explores the latest models and techniques used to study brain tumor biology. Chapters in this book are organized into four sections: in vivo models, ex vivo models, treatments in mice, and clinical imaging.

Brain Energy Metabolism addresses its challenging subject by presenting diverse technologies allowing for the investigation of brain energy metabolism on different levels of complexity. Model systems are discussed, starting from the reductionist approach like primary cell cultures which allow assessing of the properties and functions of a single brain cell type with many different types of analysis, however, at the expense of neglecting the interaction between cell types in the brain. On the other end, analysis in animals and humans in vivo is discussed, maintaining the full complexity of the tissue and the organism but making high demands on the methods of analysis. Written for the popular Neuromethods series, chapters include the kind of detailed description and key implementation advice that aims to support reproducible results in the lab.Meticulous and authoritative, Brain Energy Metabolism provides an ideal guide for researchers interested in brain energy metabolism with the hope of stimulating more research in this exciting and very important field.

This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study.

This volume provides comprehensive procedures for analyzing the extracellular matrix in native, injured, and engineered neural tissues. Divided into four parts, each focusing on different aspects of the extracellular matrix and the nervous system, Extracellular Matrix covers methods to analyze native tissue, in vitro models for investigating cell-extracellular matrix interactions in a variety of contexts, protocols to investigate the role of the extracellular matrix in nervous system injury, degeneration, and regeneration, as well as therapeutics and engineered systems. Each chapter is written by leading experts and presents established protocols in a concise format, encompassing current technologies as well as methods developed over years of research. Beginning with an introduction to the method, chapters continue with a listing of the materials and equipment, step-by-step protocols, and a Notes section with troubleshooting tips, supplemental details and alternative approaches, as well as a list of references for further information.As part of the practical and convenient Neuromethods series, Extracellular Matrix serves as an invaluable aid for researchers studying this vital area of neuroscience.

Reflecting a vast amount of new information concerning the functional characteristics of the various 5-HT receptor subtypes and the Na+-dependent serotonin transporter (SERT), this volume provides state of the art methodologies currently applied in serotonin research from leading experts in the field. Serotonin Receptor Technologies describes approaches that vary from molecular biological and biochemical techniques (e.g., regarding receptor dimerization), fluorescence microscopy and imaging applications, flow cytometry, the use of organotypic slice and cell cultures to the generation of genetically modified animal models and the development of sophisticated behavioral tests, thus covering a wide spectrum of techniques to study serotonergic signaling in detail. Written for the popular Neuromethods series, chapters include the kind of detail and practical advice that ensures successful results in the lab.Authoritative and convenient, Serotonin Receptor Technologies serves to foster both basic and translational research aiming to further deepen our understanding of the various facets of aminergic systems, as well as to aid research on similar problems with related GPCRs and neurotransmitter transporters.

To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers. Divided in five sections dedicated to experimental approaches investigating different facets of dopaminergic signal transduction, Dopamine Receptor Technologies covers epigenetic and post-transcriptional analysis, computational and biochemical techniques, visualization and imaging methods, molecular and cell biological tools, as well as behavioral assessment. The book, as a part of the popular Neuromethods series, provides insightful step-by-step protocols and methodological reviews that readers will find useful.Practical and versatile, Dopamine Receptor Technologies seeks to aid researchers in developing new pharmacological tools to improve our knowledge of in vivo roles played by each receptor subtype and the synthesis of prospective lead compounds for drug discovery.

This volume covers the latest methods used in clinical neurochemistry laboratories for both clinical practice and research. Chapters in this book discuss topics such as techniques for cerebrospinal fluid (CSF) collection, pre-analytical processing, and basic CSF analysis; an examination of biomarkers including ELISA and automated immunochemical assays for amyloid and tau markers for Alzheimer¿s disease; the analysis of neurofilaments by digital ELISA; and an example of successful novel immunoassay development. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Cerebrospinal Fluid Biomarkers is a valuable resource for clinicians and researchers to use in CSF labs and CSF courses.

This volume covers methodologies, ranging from the molecular level to the network level, used to study receptor-receptor interactions in heteroreceptor complexes inside the central nervous system. The chapters in this book cover topics such as biochemical binding techniques; receptor autoradiography; superfused synaptosome techniques; RTK-GPCR interaction; fluorescence and bioluminiscence energy transfer methods, Co-IP cytometry-based FRET; and novel bioinformatic approaches to understand membrane heteroreceptor complexes and the global panorama of their receptor-receptor interactions. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and thorough, Receptor-Receptor Interactions in the Central Nervous System is a valuable resource for any scientist or researcher interested in this field of study.

This volume provides in-depth descriptions and discussion of clinical trial designs based on novel data analysis of high-quality trials for 11 different neurological conditions in order to ultimately improve the quality of care in neurology. Some of the conditions discussed in this book are stroke, traumatic brain injury, Alzheimer¿s disease, epilepsy, and tumors of the central nervous system. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your research center and clinical investigation. Thorough and comprehensive, Clinical Trials in Neurology is a valuable guide for individuals in the research, education, and clinical practice fields, specifically in the fields of medicine, physical therapy, occupational therapy, nursing, and public health. This volume is also an important resource for clinicians aiming to improve their understanding in methodology of clinical trials.

This volume highlights various techniques used to study multiple aspects of mGlu receptor function. Chapters in this book cover topics such as modern approaches for the development and screening of allosteric modulators; fluctuations in intracellular calcium induced by mGlu receptor activation; dopamine-glutamate interactions in the central nervous system; methods for assessing mGlu receptor localization; and common techniques for evaluating mGlu modulation in behavior in rodents, including stress and alcohol drinking. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and practical, Metabotropic Glutamate Receptor Technologies provides researchers with a starting point to address important questions regarding mGlu and its importance. Researchers working in the fields of neuroscience and physiology of multiple peripheral systems will find this volume most useful.

This volume explores the recent advances in the study of translational paths in central inflammation and focuses on ongoing pathophysiological processes and the transition between inflammatory stages and progressive states with neurodegeneration. Chapters cover topics such as pathophysiological hallmarks of neuroinflammation from tissue damage to reorganization; connecting studies of mouse models; and investigations of humans with multiple sclerosis. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Translational Methods for Multiple Sclerosis Research is a valuable resource for researchers who want to learn more about this chronic ad progressive disease, and pave the way for new advancements.

This volume presents a wide array of animal models that are used in the study of the individual and environmental factors involved in the development of addiction, the consequences of chronic drug exposure, and recently developed behavioral and biological procedures for the treatment of addictive disorders. The chapters in this book are organized into three sections that cover the aforementioned aspects in relation to different drugs of abuse and pathological gambling. The first section of the book focuses on the individual variables that affect vulnerability to addiction to nicotine, alcohol, cocaine, psychostimulants, and gambling. Section Two looks at the environmental variables that contribute to the vulnerability to addiction. Section Three explores the consequences of chronic drug consumption and new approaches to the treatment of addiction. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successfulresults in your laboratory.Cutting-edge and comprehensive, Methods for Preclinical Research in Addiction is a valuable resource that helps researchers and scientists understand the neurobiological mechanisms that underlie the influence of individual and environmental variables on the risk for addiction, and assists them in developing new behavioral and pharmacological strategies to prevent and treat addictive disorders.

This open access volume provides an overview of the latest methods used to study neuronal function with all-optical experimental approaches, where light is used for both stimulation and monitoring of neuronal activity. The chapters in this book cover topics over a broad range, from fundamental background information in both physiology and optics in the context of all-optical neurophysiology experiments, to the design principles and hardware implementation of optical methods used for photoactivation and imaging. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, All-Optical Methods to Study Neuronal Function is a valuable resource for researchers in various disciplines such as physics, engineering, and neuroscience. This book will serve as a guide to establish useful references for groups starting out in this field, and provide insight on the optical systems, actuators, and sensors. This is an open access book.

This volume discusses the latest experimental and clinical approaches to study seizures and epilepsy. Chapters in this book cover detailed protocols for ex vivo and in vitro experimental models and widely-used in vivo (acute and chronic seizure) models in epilepsy research.