Bøger i Practical Manual Series serien

The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.

The present book embodies 15 s viz. Preparation of different liquid and solid media, isolation techniques for soil, air, water and seed borne microbes, micrometry, microbial growth, Ames test, assay of antibiotics, antibiotics sensitivity test, bacterial transformation, IMVIC test, growth curve of coliphage, culture techniques from body fluids, acid and alcoholic fermentation and each of which includes protocol of several microbiological practicals prescribed in the syllabi of graduate and post graduate courses of microbiology, biochemistry and biotechnology. Besides these, the book also contains the methods of qualitative and quantitative analysis of air, soil and water borne pathogenic and non-pathogenic microbes and agriculturally important microbes. For the convenience and better understanding, most of the methods have been described with help of flow chart and sketches. Authors are confident that the present book will certainly be very useful to learn and handle different type of microbiological experiments in the laboratory.

"Cover methods used in clinical examination of herd/individual animals including history taking related to production disease of animals and laboratory for confirmatory diagnosis of disease. This book covers all practical aspects of clinical examination of animal and most commonly used laboratory techniques (including ruminal fluid examination, CSF examination, bone marrow and lymph node biopsy, blood transfusion, enzymology and other biochemical parameters) for the diagnosis of metabolic/production diseases. Questions given at the end of each , interpret and correlate the results with clinical signs of the diseases, which can be an aid to the confirmatory diagnosis of disease."

"Biotechnology: laboratory manual provides basic protocols required for students of undergraduate and postgraduate programme. The protocols are explained in a simplified manner and are very easy to conduct. The book is a collection of experiments from all fields of biotechnology and will become a companion for all those who do research in the field of biotechnology. Attention is given to include most of the basic protocols. This book will provide first hand valuable information for all those who are interested in biotechnology research."

The book is ready reference practical manual for teachers as well as students of undergraduate programmes and postgraduate beginners in the discipline of horticulture and postharvest technology. Spread over 33 s, the book covers various topics i.e. acquaintance with equipments and machinery, maturity indices, pre-storage treatments, low cost storage technology, processing and value addition in fruits and vegetables including preparation of jam, jelly, marmalade, beverages, pickles, chutney, tomato products, canning of fruits and vegetables, cut out analysis and preparation of cider, vase life of flowers, physico-chemical and sensory analysis of fresh & processed products. Besides, ten annexures have also been given describing fruit products order, temperature corrections for measuring TSS, maturity indices, units of conversion, preparation of cleaning and chemical solutions, food additives, suppliers of machinery and equipments, important journals and conduct of a students in a laboratory. The contents of s have been put up in the simplest language giving separate instructions for the students and teacher as well as relevant information on the topics so that conduct of practical becomes easy and systematic. Coloured plates of some of the machinery and equipments have also been given so that their identification becomes easy even if the equipment or machinery is not available in a particular laboratory. The book is an initiative to fulfill the long pending requirement of teachers and students for conduct of practical s on various aspects of postharvest technology of horticultural crops. The book would be of great use to the students, researchers, teachers and all those who have interest in the subject.