Bøger i Practical Manual Series serien

"Biotechnology: laboratory manual provides basic protocols required for students of undergraduate and postgraduate programme. The protocols are explained in a simplified manner and are very easy to conduct. The book is a collection of experiments from all fields of biotechnology and will become a companion for all those who do research in the field of biotechnology. Attention is given to include most of the basic protocols. This book will provide first hand valuable information for all those who are interested in biotechnology research."

"Cover methods used in clinical examination of herd/individual animals including history taking related to production disease of animals and laboratory for confirmatory diagnosis of disease. This book covers all practical aspects of clinical examination of animal and most commonly used laboratory techniques (including ruminal fluid examination, CSF examination, bone marrow and lymph node biopsy, blood transfusion, enzymology and other biochemical parameters) for the diagnosis of metabolic/production diseases. Questions given at the end of each , interpret and correlate the results with clinical signs of the diseases, which can be an aid to the confirmatory diagnosis of disease."

The present book embodies 15 s viz. Preparation of different liquid and solid media, isolation techniques for soil, air, water and seed borne microbes, micrometry, microbial growth, Ames test, assay of antibiotics, antibiotics sensitivity test, bacterial transformation, IMVIC test, growth curve of coliphage, culture techniques from body fluids, acid and alcoholic fermentation and each of which includes protocol of several microbiological practicals prescribed in the syllabi of graduate and post graduate courses of microbiology, biochemistry and biotechnology. Besides these, the book also contains the methods of qualitative and quantitative analysis of air, soil and water borne pathogenic and non-pathogenic microbes and agriculturally important microbes. For the convenience and better understanding, most of the methods have been described with help of flow chart and sketches. Authors are confident that the present book will certainly be very useful to learn and handle different type of microbiological experiments in the laboratory.

The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.