Bøger udgivet af Cold Spring Harbor Laboratory Press

Mosquitoes have been studied for centuries by entomologists, epidemiologists, biologists, geneticists, and ecologists. Early investigations primarily focused on their anatomy, life cycles, and feeding habits. As scientific knowledge progressed, however, the research scope expanded and so has our understanding of the underlying biology of mosquito genetics, physiology, behavior, and pathogen transmission dynamics. This laboratory manual provides a comprehensive collection of experimental procedures for research using mosquitoes. It includes basic methods for mosquito rearing, egg collection, scoring mating status, scoring reproductive and host-seeking behaviors, visualizing transcript and protein expression, and evaluating physiological responses to stimuli. There are also protocols for genetic manipulation of mosquitoes using RNAi and CRISPR-Cas9 genome editing. Methods to evaluate insecticide resistance are also described. The methods in this manual focus primarily on Aedes and Anopheles mosquitoes, although we also provide rearing protocols for Culex, and many of the protocols included may also work well in multiple species, with minor modifications. This manual aims to serve both as a critical reference for laboratories already working with mosquitoes and as a comprehensive guide for those starting to use mosquitoes in their experimental work.

"This book is about the history of emerging new therapies and preventions for a host of human diseases via biotechnology; the science that drives it and the people and companies who transformed that science into the biotechnologies we benefit from"--

"This volume summarizes the proceedings of two workshops sponsored by the Bill and Melinda Gates Foundation on cutting edge systems and synthetic biology research which could facilitate mitigation and adaptation through quantum leaps in photosynthesis, fertilization, methane control, and drought and disease resistance, and thereby substantially increase crop yield and soil carbon. The technologies are at various stages of development--some are ready for field testing while others are still in the laboratory, but those that pass proof of principle should be available and implementable within a decade. A corollary benefit might also be the activation of economic incentives to regard CO2 and CH4 as potential resources rather than wastes; this could lower the volume of unproductive arguments over "whose fault it is" that greenhouse gases have increased rapidly in recent times"--

The African turquoise killifish Nothobranchius furzeri is a short-lived fish species that has been established as a novel vertebrate model to study aging and represents a promising system to examine various other processes. The killifish is relatively easy and inexpensive to maintain. In addition, it has a short life cycle and abundant progeny, facilitating its use. Investigation using this model organism is growing and has provided new insights into aging, age-related diseases, ecology, evolution, and embryonic diapause--a state of "suspended animation." This laboratory manual provides a comprehensive collection of protocols covering experimental techniques currently used to study the African turquoise killifish. Husbandry protocols describe how to set up and maintain a killifish colony and how to generate and preserve genetically engineered killifish lines. This collection also includes both general and specific methods to characterize different organs during aging, with a particular focus on the brain. Several genomics methods and analyses for unbiased characterization are also described. Finally, this manual encompasses protocols in other interesting areas of research, including injury repair, development, and embryonic diapause. This collection should enable studies in the African turquoise killifish and other species of killifish such as the South American killifish, and includes protocols that will be useful both to groups considering the use of these systems for the first time and to those with experience in the field. We also hope that this protocol book will foster a large killifish community and will promote new studies in many different research areas.

Over 80 investigators provide a panoramic account of how cells control and repair the folding of newly synthesized proteins and transport them correctly to membranes, mitochondria and other cellular addresses. This volume should be useful to all cell biologists. It also provides in-depth background information for specialists in synaptic function, cell movement, antigen presentation and other cellular functions to which protein kinesis is vital.

This book is an easy-to-follow handbook that introduces readers to entry-level clinical job opportunities and explains how to qualify for them, with particular emphasis on how to gain "clinical experience" that a hiring manager will accept.

The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done.

Members of the frog Xenopus genus are versatile model organisms used in developmental biology, cell biology, neuroscience, and toxicology research, among others. Frogs are easy to rear and maintain, and large sample sizes of embryos and later stages are readily obtained. Studies using Xenopus cell-free extracts, oocytes, eggs, embryos, larval stages, and adult frogs have yielded important insights into a multitude of key biological processes from mechanisms underlying the cell cycle to embryonic development to human disease. This new laboratory manual provides a comprehensive collection of experimental procedures for research using Xenopus. It includes basic methods for Xenopus husbandry, egg harvesting, embryo collection, microdissection, and visualization, as well as strategies for extract preparation, genetic manipulation, genomic analysis, transcriptomics, and proteomics. There are protocols for analyses of Xenopus immunology, neurobiology, metamorphosis, and adult physiology. Toxicological studies and chemical screening methods are also included. The manual covers both Xenopus laevis and the smaller Xenopus tropicalis species. It is a critical reference for laboratories that currently work with these organisms and will serve as an essential start-up guide for those seeking to start using Xenopus frogs in their research for the first time.

The Proceedings of the 79th Symposium capture many of the tremendous discoveries currently being made by neuroscientists and psychologists working on cognitive processes at scales varying from the molecular to circuit to whole-brain and theoretical studies. Major advances in understanding cognitive function, including learning, memory, language, and decision-making, are being made through the application of powerful new techniques and approaches. The scientific results discussed in this volume shed light on many areas of normal brain function but also offer novel insights into the treatment of psychiatric, neurological, and neurodegenerative diseases. This volume includes state-of-the-art neurobiological research at genetic, molecular, cellular, circuit, and whole brain resolution. Additional chapters address computational neuroscience, behavioral and psychophysical studies, and evolutionary/comparative neuroscience. The Conversations included in this volume are based on interviews conducted during the Symposium and offer a broader anecdotal perspective on this fascinating subject by many of the world's leading cognitive neuroscientists. The Symposium Proceedings provides an integrative overview of the current state of the field as well as a glimpse into the emerging connections between molecular/cellular /circuit mechanisms and higher brain function by leading authorities in the field.

Bacteria are powerful systems for genetic research. They are fast growing and easy to propagate and maintain, and many tools have been developed for their genomic analyses and genetic manipulation. The study of bacteria and their viruses allowed deciphering the genetic code and has shaped our understanding of fundamental biological processes such as DNA replication, transcription, and translation. This manual provides a collection of experimental procedures for genetic research in bacteria. It describes basic methods for the isolation and propagation of bacteria and phages, purification of genomic DNA and plasmids, and introduction of DNA into bacteria. Diverse strategies for generating and confirming mutations and for creating gene deletions and gene fusions are described, using allelic exchange, random transposition, recombineering, and CRISPR-Cas technologies. Protocols for bacterial genome sequencing and resequencing, as well as for high-throughput mutant screening via transposon sequencing, are provided. Although specific bacterial species such as Escherichia coli, Salmonella enterica, Vibrio cholerae, and Staphylococcus aureus are used for some of the protocols, the methodologies herein described can readily be adapted for use with other bacteria. This manual thus represents a valuable resource for those seeking to perform genetic experiments in bacteria.

Grounds for Knowledge is an engaging and knowledgeable guide to CSH Laboratory's buildings both historic and new, and to the 150 species of trees that surround them. The superb color photography and detailed maps invite exploration of the newly designated Bungtown Botanical Garden. Buildings and landscapes of nearby Lab campuses in Woodbury, Lloyd Harbor, and Cold Spring Harbor are covered as well.

This book tells the story of how mRNA's medical potential was finally realized, setting the stage for a coming revolution in which our own bodies will generate therapeutic molecules we need. mRNA was long overlooked by mainstream molecular biologists. The pathway to recognition of its therapeutic possibilities was littered with broken careers, lawsuits, and opportunities missed by pharmaceutical companies.

The retina is a layer of neural tissue that lines the inner eye and captures visual stimuli. This volume provides an update on retinal diseases, describing the genetic and pathophysiological basis of the diseases and advances in strategies to treat them.

Identified more than a hundred years ago by Camillo Golgi, the Golgi is a series of flattened sacs that acts as a critical sorting and processing station for intracellular traffic. This volume covers recent insights into Golgi architecture, mechanisms of trafficking through the Golgi stacks, and more.

The gene has become a cultural icon and an increasingly rich source of imagery and ideas for visual artists. Drawing on a wide range of contemporary painting and sculpture, The Molecular Gaze: Art in the Genetic Age explores the moral and bioethical questions these works address. What does it mean to be human? What is "identity" in a society of genetically manipulated individuals? Questions like these are growing louder as genetic technology advances and the public examines the ethical consequences more widely. Suzanne Anker and Dorothy Nelkin, an artist and a social scientist, have written a thought-provoking and visually fascinating book for scientists, artists, students, and general readers intrigued by the anxiety and exhilaration of the genetic age.

This book, which includes the first fourteen chapters of the acclaimed monograph DNA Replication in Eukaryotic Cells, is a broad account of the basic principles of DNA replication and related functions such as DNA repair and protein phosphorylation. It also includes a newly commissioned chapter in which the editor, Mel DePamphilis, provides a review of the most recent advances in understanding the complex gene and protein interactions that underpin this essential cellular function.