Bøger udgivet af Springer US

This volume covers the latest advancements and techniques used to understand the fastidious bacterium, Borrelia burgdorferi, and its significance in infectious disorders by combining both conventional and cutting-edge approaches. This book covers diverse topics, including direct detection, diagnostic methods, immune response analysis, alternative model systems, advanced proteomics, social media analysis, and clinical research. It also discusses unconventional wet lab research such as content analysis, the use of ChatGPT, clinical algorithms for chronic Lyme, establishment of a pregnancy Lyme disease biobank, and investigates Lyme in pregnant women. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Borrelia burgdorferi: Methods and Protocols encompasses a wide range of techniques and caters to scientists from various disciplines and career stages, such as cell and molecular biologists, statisticians, and clinical researchers.

This detailed volume explores a wide range of evidence-based complementary medicine and various bio-analytical techniques used to define botanical products. Collecting recent work and current developments in the field of contemporary phytomedicine as well as their future possibilities in human health care, the book includes unique contributions in the form of chapters on phytomedicine and screening biological activities explained with diverse hyphenated techniques, as well as issues related to herbal medications, such as efficacy, adulteration, safety, toxicity, regulations, and drug delivery. Written for the Springer Protocols Handbooks series, chapters feature advice from experts on how to best conduct future experiments. Extensive and practical, Natural Product Experiments in Drug Discovery serves as an ideal reference for students, professors, and researchers in universities, R&D institutes, pharmaceutical and herbal enterprises, and health organizations.

This volume explores multiple methods and approaches used to generate human brain and neuroretinal organoids to address fundamental questions in human brain research. The chapters in this book cover topics such as self-organizing brain organoids with ventricles amenable to injection and electroporation; visualization of 3D organoids though the latest advancements in microscopy; generation of 3D retinal tissue with physiologically competent, light-sensitive photoreceptors; modeling brain tumors using genetically edited brain organoids; and brain organoids as a model to study Zika virus and SARS-CoV-2 infections. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.  Comprehensive and cutting-edge, Brain Organoid Research is a valuable resource for researchers at various levels of learning, ranging from undergraduate students, early researchers, and advanced laboratories. This book aims to be instrumental in moving this developing field forward.

This volume details experimental protocols to study structure and function of hepatocytes in vivo and in vitro, and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte subfractions, characterizing the heterogeneity, novel culture systems, hepatocyte-like cells from human pluripotent stem cells, analyzing drug metabolism, monitoring protein sorting, manipulating gene expression, and methods for expanding primary hepatoblastoma and identifying hepatocellular carcinoma subtypes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Hepatocytes: Methods and Protocols aims to provide techniques to further understand molecular mechanisms involved in the regulating hepatocyte functions and underlying liver diseases.

This updated volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and limited need for specialized reagents. These techniques are essential in laboratory fields including endocrinology, biochemical genetics, drug analysis, proteomics, and pathogen identification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols, Second Edition is an ideal resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.

This volume provides an overview of well-established methods optimized for diverse archaeal model organisms and is a source of protocols facilitating access to the molecular and cellular biology characterization of these fascinating organisms. Chapters are divided into five parts detailing available genetic tools, molecular and cellular biology methods, strategies to study the ecophysiology of archaea, and classroom protocol. Each main thematic part is also introduced by future-oriented and authoritative primers. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Archaea: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

This book provides insight into the biology of Wolbachia with a particular focus on methodology. Chapters guide readers through reproductive phenotypes caused by Wolbachia, survival and replication of Wolbachia in insect cell lines, artificial introduction of Wolbachia into novel hosts, and analysis of Wolbachia gene functions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

This volume provides detailed protocols for the isolation, enumeration, characterization of diverse bacteriophages, including both small and jumbo bacteriophages, from soil, fecal, municipal wastewater, and from food niche samples. Chapters highlight the diversity of bacteriophages in different environments, quantifications using culture and molecular techniques, protocols for isolation, interaction of bacteriophage proteins with host cells, and how to use bacteriophages to transfer foreign genetic elements to bacterial strains. In addition to the above, chapters feature the application of bacteriophages/bacteriophage-derived products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips (in the Notes section) on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacteriophages: Methods and Protocols aims to ensure successful results in further study of this vital field.

This volume provides a comprehensive compilation of protocols in T cell repertoire analysis, from the leading experts in the field, representing both well-established methods and cutting-edge advances. Chapters broadly cover the emerging new T cell subsets, sequencing technologies for capturing TCR repertoire, and computational tools for analyzing an ever-growing TCR repertoire, with a particular focus on how to link the sequence with TCR antigen specificity. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Cell Repertoire Characterization aims to be a useful practical guide to researches to help further their study in this field. 

This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a wide range of biological systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fluorescent Proteins: Methods and Protocols serves as an ideal guide for students and academicians enthusiastic about the recent progress in the practical application of fluorescent protein technology.

This volume provides backgrounds, methods, and troubleshooting for the analysis of circadian rhythms at the molecular, cellular, and organismal levels in vertebrates and invertebrates, and also covers light input, sleep, circalunar rhythms, and seasonal rhythms. The chapters in this book discuss behavioral and biological testing, histochemistry, single-cell imaging, electrophysiology, photochemistry, genetics, genomics, transcriptomics, proteomics, metabolomics, systems biology, chemical biology, and mathematical modeling. In the Neuromethods series style, chapters include experimental detail and key advice from the specialists needed to successfully carry out these methods in your laboratory. Cutting-edge and practical, Circadian Clocks is a valuable resource for all researchers seeking to learn about established and novel methods in chronobiology, and the molecular mechanisms of the rhythmic control of physiology and behavior. 

This volume discusses methods used in the fields of molecular and cellular biology for detecting and studying cell death, especially in cancer and cancer therapy. Chapters in this book cover topics such as non-destructive, real-time Annexin V apoptosis assay; assessment of the immune response to tumor cell apoptosis and efferocytosis; mass cytometry assessment of cell phenotypes and signaling states in human whole blood; cell cycle analysis of ER stress and autophagy; and simultaneous detection of inflammasome activation and membrane damage during pyroptosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Apoptosis and Cancer: Methods and Protocols is a valuable resource and practical guide for both novice and expert researchers looking into the "e;meaning of death."e;

This fully updated volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Exploring the versatility and flexibility of mass spectrometry, the book covers the advantages of this technology, which typically include elimination of the need for special reagents such as antibodies, increased sensitivity and specificity, and multi-component analysis enabling the screening of tens to hundreds of compounds in a single assay run. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Clinical Applications of Mass Spectrometry in Drug Analysis: Methods and Protocols, Second Edition serves as a valuable resource for laboratory professionals who arealready utilizing mass spectrometry or considering bringing this technology to their labs.

This volume looks at the latest advancements used by researchers to study psychophysiology and cognitive neurosciences. The chapters in this book cover topics such as classical event-related brain responses (P300, MMN, and CNV); anatomical structures and physiological mechanisms underlying the capability of feeling pain and smelling; magnetoencephalography (MEG); and brain-computer interface techniques using electrical activity generated by the brain. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Psychophysiology Methods is a valuable resource for researchers who want to learn more about the use of psychophysiological techniques in the investigation of human cognition, and increase interest in the clinical puzzle of neurological and psychiatric disorders.

This second edition volume expands on the previous edition with new and updated chapters that highlight the latest methods and approaches for the manipulation of RNA viruses. The chapters in this book explore the fundamental role in studying RNA viruses; identifying markers of host range, disease, and transmission; and aid readers with the further development of in silico computational biology tools and artificial intelligence algorithms that can help predict the emergence of certain pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Reverse Genetics of RNA Viruses: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this developing field.

This detailed volume explores a variety of techniques used to study lineage commitment in stem cells. Further elucidation of the process that stem cells undergo on their way to becoming more specified cell types is vital for a more complete understanding of cell biology and overall physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cells and Lineage Commitment: Methods and Protocols serves as an ideal guide for experts and novices in the field of stem cell biology.

This volume explores the latest techniques used to study environmental microbial evolution, with a focus on methods capable of addressing deep evolution at long timescales. The chapters in this book are organized into three parts. Part One introduces molecular dating approaches and time calibration ideas that allow for the determination of evolutionary timescales of microbial lineages. Part Two describes several advanced phylogenomic tools such as models for genome tree construction, a taxon sampling method, outgroup-independent tree-rooting methods, and gene family evolution models. Part Three covers techniques used to study trait evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Environmental Microbial Evolution: Methods and Protocols is a valuable tool for all researchers who are interested in learning more about this important and evolving field. 

This second edition expands on the previous volume by incorporating state-of-the-art electrophysiological and anatomical methods and their application to the study of several systems of the brain involved in a range of functions. Chapters in this edition cover topics such as the value and difficulty of multi-site recordings using depth or surface electrodes; an assessment of different electrophysiological techniques used in non-human animals and humans; applying single-unit and ensemble recordings to the study of temporal dynamics in cognition; approaches to electroencephalography (EEG) recordings applied to mouse research; recordings of the nucleus reuniens of the ventral midline thalamus; and a comprehensive account of intracranial electroencephalography performed in patients with drug resistant epilepsy. In the Neuromethods series style, chapters include the type of detail and key advice from specialists needed to obtain successful results in your laboratory. Comprehensive and thorough, Electrophysiological Recording Techniques, Second Edition is a valuable resource for researchers and clinicians to help them utilize the principles and research designs described herein in their programs.  

This volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries; DNA encoding of chemical structure; design, preparation and analysis of target proteins and tool compounds; screening of soluble protein targets by affinity selection; DEL qPCR, preparative PCR and DNA sequence analysis; computational methods used to analyze selections and choose compounds for resynthesis; and analysis of hit compounds. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, DNA-Encoded Chemical Libraries: Methods and Protocols is a valuable resource for scientists interested in DEL technology for drug discovery, and will contribute to the continued advancement in this important field.

This new edition offers a state-of-the-art and integrative vision of pharmacogenomics by exploring new concepts and practical methodologies focusing on disease treatments, from cancers to cardiovascular and neurodegenerative disorders and more. The collection of these theoretical and experimental approaches facilitates problem-solving by tackling the complexity of personalized drug discovery and development. Written by leading experts in their fields for the highly successful Methods in Molecular Biology series, the book aims to provide across-the-board resources to support the translation of pharmacogenomics into better individualized health care. Authoritative and up-to-date, Pharmacogenomics in Drug Discovery and Development, Third Edition aims to aid researchers in approaching the challenges in pharmacogenomics and personalized medicine with the introduction of these novel ideas and cutting-edge methodologies.

This volume provides current state-of-the-art methods for screening, diagnosis, management, and prevention of fetal alcohol spectrum disorder (and FASD). Chapters detail animal models, mechanism of ethanol teratogenesis, genetic mechanisms, clinical perspectives, physical traits in children with FASD, managing behavior, FASD and the correction system, nutritional influences aiding FASD prevention, prenatal alcohol screening, the use of dental signatures in FASD diagnosis, the experiences of FASD research and clinical practices in Australia, Canada, UK, USA, adult FASD diagnosis, FASD and children in care, FASD and the healing journey.Authoritative and cutting-edge, Advances in Fetal Alcohol Spectrum Disorder aims to be a useful practical guide to researchers and clinicians to help further their study in this field.

This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Optical Tweezers: Methods and Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.

This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome concepts, such as epigenetic and its cause of cancer are presented. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chromosome Analysis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

This volume covers the latest advances in technologies that look at familial cardiomyopathies in greater detail, and provides new computational and experimental models that model, study, and detect disease at earlier stages. Together, this allows interdisciplinary research experiments to provide new insights for the development of novel interventions that slow, stop, or even reverse the disease process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Familial Cardiomyopathies: Methods and Protocols aims to inspire further development of techniques used to study myocardial disease and the development of new, sarcomere-targeted therapeutic approaches for the maintenance of heart health and the treatment of heart failure.

This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products. Authored by leading experts and written in the highly successful Methods in Molecular Biology series format, each chapter includes introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and valuable tips for troubleshooting and avoiding known pitfalls.Whether you are a seasoned scientist or a newcomer, Bacteriophage Therapy: From Lab to Clinical Practice, Second Edition provides essential tools and knowledge to advance bacteriophage research and its translation into innovative medicinal products and clinical therapies.

This volume covers a broad range of cell types including cultured cell lines, primary cells, and various unicellular organisms such as fission yeast, budding yeast, parasite Leishmania amazonensis, and parasite Trypanosoma brucei. The chapters in this book are organized into four parts. Part One looks at a general overview of cell cycle control and synchronization. Part Two discusses techniques to synchronize mammalian cells to various cell cycle phases including mitotic sub-phases. Part Three covers synchronization of unicellular organisms and Part Four analyzes cell cycle progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cell-Cycle Synchronization: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.

This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field. Chapter "e;Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo"e; is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This volume discusses the latest techniques used in the diverse fields of single cell 'omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory. Cutting-edge and comprehensive, Single Cell 'Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.

This second edition volume expands on the previous edition with discussions about the latest viral metagenomics aspects covering a range of different specimens such as soil, freshwater, wastewater, fecal samples, blood plasma, clinical tissues, fungi, and herbarium samples. Chapters also look at different viral groups including archaeal viruses, eukaryotic viruses, phages, mycoviruses, and circular DNA viruses. Techniques required for studying the three viral metagenomic steps of samples processing, library construction, and analysis of data are also discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Vital Metagenomics: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this important and developing field.

This book describes state-of-the-art approaches for identification and functional characterization of plant peptide hormones and growth factors. Chapters guide readers through protocols for computational identification of novel signaling peptides and phytocytokines, analysis of post-translational maturation events, isolation of mature peptides from plant tissues, peptide bioassays, in vivo and in vitro analysis of peptide activity, peptide receptor identification and interaction analysis, and quantitative assessment of peptide ligand binding to cognate receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Peptide Hormones and Growth Factors aims to be a useful and practical guide to new researchers and experts looking to expand their technical know-how.