Udvidet returret til d. 31. januar 2025

Comparative Study of Ivf and Parthenogenesis in Bubaline Oocytes

Bag om Comparative Study of Ivf and Parthenogenesis in Bubaline Oocytes

The objective of this study was to understand the pitfalls of the poor oocyte cleavage and subsequent embryo production by assessing the developmental competence of the oocytes in buffalo using parthenogenesis as a model which was compared with IVF (natural activation). Buffalo ovaries were collected from slaughter house and matured oocytes were subjected to either IVF (control) or chemical activation (treatment)[7% ethanol (ET)for 7 min + 2.5 mM 6-Di methyl amino purine (6-DMAP) for 4 hr, or 7% ET for 7 min + 10 ug/ml Cycloheximide (CHX) for 6 hr or 7% ET for 7 min + 2.5 mM 6-DMAP + 10 ug/ml CHX for 6 hr]. Fertilized and chemically activated oocytes were cultured in mSOF medium for 8 days to study embryo development.In conclusion, the results of parthenogenesis, revealed that buffalo oocytes had better inherent developmental competence and the poor cleavage and embryo development following IVF, in our study, may partly be due to the poor quality of frozen-thawed sperm, improper sperm capacitation and/ or fertilization. Further, high ambient temperature adversely affected the cleavage and embryo development following IVF.

Vis mere
  • Sprog:
  • Engelsk
  • ISBN:
  • 9783844326963
  • Indbinding:
  • Paperback
  • Sideantal:
  • 140
  • Udgivet:
  • 6. april 2011
  • Størrelse:
  • 152x229x8 mm.
  • Vægt:
  • 213 g.
  • BLACK NOVEMBER
Leveringstid: 2-3 uger
Forventet levering: 23. november 2024

Beskrivelse af Comparative Study of Ivf and Parthenogenesis in Bubaline Oocytes

The objective of this study was to understand the pitfalls of the poor oocyte cleavage and subsequent embryo production by assessing the developmental competence of the oocytes in buffalo using parthenogenesis as a model which was compared with IVF (natural activation). Buffalo ovaries were collected from slaughter house and matured oocytes were subjected to either IVF (control) or chemical activation (treatment)[7% ethanol (ET)for 7 min + 2.5 mM 6-Di methyl amino purine (6-DMAP) for 4 hr, or 7% ET for 7 min + 10 ug/ml Cycloheximide (CHX) for 6 hr or 7% ET for 7 min + 2.5 mM 6-DMAP + 10 ug/ml CHX for 6 hr]. Fertilized and chemically activated oocytes were cultured in mSOF medium for 8 days to study embryo development.In conclusion, the results of parthenogenesis, revealed that buffalo oocytes had better inherent developmental competence and the poor cleavage and embryo development following IVF, in our study, may partly be due to the poor quality of frozen-thawed sperm, improper sperm capacitation and/ or fertilization. Further, high ambient temperature adversely affected the cleavage and embryo development following IVF.

Brugerbedømmelser af Comparative Study of Ivf and Parthenogenesis in Bubaline Oocytes



Find lignende bøger
Bogen Comparative Study of Ivf and Parthenogenesis in Bubaline Oocytes findes i følgende kategorier:

Gør som tusindvis af andre bogelskere

Tilmeld dig nyhedsbrevet og få gode tilbud og inspiration til din næste læsning.